工作职责
职位概述 我们正在招聘一位积极主动、注重细节的实验室技术员(Lab Technician),具备扎实的湿实验技术背景,以支持我们的实验和转化研究项目。该职位将涉及细胞培养、RNA 提取、样本管理及实验室日常维护。理想的候选人能够在快节奏的环境中高效工作,重视实验的准确性,并希望为下一代 RNA 治疗药物的研发做出直接贡献。 主要职责 实验操作
- 在 BSL-2 条件下分离并培养外周血单个核细胞(PBMCs)。
- 使用柱基或磁珠方法进行 RNA 提取。
- 使用 Qubit、NanoDrop 和 TapeStation 检测核酸质量。
- 严格遵循无菌操作准备试剂、缓冲液和培养基。 仪器操作与维护
- 操作并校准常规实验室设备,包括离心机、PCR 仪、生物安全柜和自动化液体处理器。
- 执行日常维护,记录设备使用日志,并在需要时协调设备维修。 样本与数据管理
- 接收、标记、跟踪并存储生物样本,使用实验室信息管理系统(LIMS)。
- 记录实验元数据,并整理实验结果供计算生物学团队使用。 质量与合规
- 遵循内部标准操作流程(SOP)、GLP 准则及环境健康与安全(EH&S)要求。
- 参与内部审计,并为实验流程的持续优化做出贡献。 团队支持
- 监控并补充实验室耗材,及时下单以避免停工。
- 参与每日团队会议,清晰汇报实验进展及问题。 POSITION SUMMARY We are seeking a highly motivated and detail-oriented Lab Technician with a strong background in wet-lab techniques to support our experimental and translational research programs. This role will involve hands-on cell culture, RNA preparation, sample tracking, and laboratory maintenance. The ideal candidate is someone who thrives in a fast-paced environment, values experimental precision, and wants to contribute directly to the development of next-generation RNA-based therapeutics. KEY RESPONSIBILITIES Bench Operations
- Isolate and culture peripheral blood mononuclear cells (PBMCs) under BSL-2 conditions.
- Perform RNA extraction using column-based and magnetic-bead protocols.
- Assess nucleic acid quality using Qubit, NanoDrop, and TapeStation.
- Prepare reagents, buffers, and media with strict adherence to sterile technique. Instrumentation & Maintenance
- Operate and calibrate standard lab equipment, including centrifuges, thermal cyclers, biosafety cabinets, and automated liquid handlers.
- Perform routine maintenance, maintain instrument logs, and coordinate service visits as needed. Sample & Data Management
- Receive, label, track, and store biological specimens using a Laboratory Information Management System (LIMS).
- Record experimental metadata and organize data for the computational biology team. Quality & Compliance
- Follow internal Standard Operating Procedures (SOPs), GLP guidelines, and EH&S requirements.
- Participate in internal audits and contribute to the continuous improvement of protocols. Team Support
- Monitor and replenish laboratory consumables; place orders to avoid downtime.
- Participate in daily team meetings and communicate experiment progress and issues clearly.
任职要求
- 生物学、生物化学、生物技术或相关生命科学领域本科及以上学历。
- 至少 3 年实验室工作经验(学术或工业环境均可)。
- 熟悉细胞培养、RNA 提取及常规分子生物学技术。
- 拥有出色的实验记录和组织能力。
- 能够独立工作并具备良好的团队协作能力。
- 能够长时间站立,并在需要时搬运 11 公斤以内的实验物资。 加分技能
- 具备大规模 RNA 测序(bulk RNA-seq)相关经验。
- 拥有良好的分析思维、问题解决和科研沟通能力。
- 熟悉项目管理软件和工具。
- Bachelor's degree or higher in Biology, Biochemistry, Biotechnology, or a related life science field.
- At least 3 years of hands-on experience in a laboratory setting (academic or industry).
- Familiarity with cell culture, RNA extraction, and general molecular biology techniques.
- Excellent record-keeping and organizational skills.
- Ability to work independently and in a team-based environment.
- Able to stand for extended periods and lift laboratory supplies up to 11 kg as needed. DESIRABLE SKILLS
- Experience with bulk RNA-seq analysis.
- Strong background in analytical thinking, problem-solving, and scientific communication.
- Familiar with project management software and tools.