We are seeking a passionate and results-driven Senior Scientist with expertise in challenging protein targets, biophysical characterization, and structural biology. The successful candidate will advance our mechanistic understanding of challenging protein targets to support structure-based drug discovery.
Key Responsibilities
- Design, execute, and optimize experiments for the expression and purification of recombinant proteins using
E. coli
, yeast, insect, and mammalian expression systems. - Develop and implement innovative strategies to improve protein yield, purity, stability, and functionality, particularly for membrane/lipid-associated or aggregation-prone proteins.
- Characterize protein properties and interactions using biophysical methods (e.g., CD, DSF, MALS, MST, BLI, SPR).
- Support Cryo-EM and X-ray crystallography efforts for structure determination, model building, and mechanistic interpretation.
- Apply computational tools such as protein modeling, molecular docking, and structure analysis to integrate experimental and structural data and guide compound optimization.
- Analyze and interpret experimental data, prepare technical reports, and present findings to project teams.
Qualifications
- Ph.D. in Protein Biochemistry, Structural Biology, Biochemistry, Biophysics, or related fields.
- Minimum 5 years of hands-on experience in recombinant protein expression, purification, and structural or biophysical analysis; 1–2 years of industrial experience preferred.
- Strong background in multiple expression systems (
E. coli
, yeast, insect, and/or mammalian) and diverse purification methods (affinity, ion exchange, size exclusion chromatography, etc.). - Demonstrated experience in Cryo-EM or X-ray crystallography for structural determination.
- Familiarity with computational modeling and molecular docking tools is highly desirable.
- Proven analytical and troubleshooting skills, scientific curiosity, and the ability to work both independently and collaboratively in a fast-paced environment.
- Ability to follow and critically evaluate emerging literature and design experiments aligned with project goals.